Abstract
SUMMARY: BACKGROUND: In Type 1 Diabetes Mellitus (T1DM), numerous changes in the cellular as well humoral immune response have been identified. However, it is not known whether both the CD4+ and CD8+ subpopulation or only one of these or CD19+contains increased numbers of activated cells. OBJECTIVE: The aim was to study the activated lymphocyte subpopulation by use of monoclonal antibodies to T-cell and B-cell antigens which is known to be expressed on activated cells. METHODS: A total of 60 T1DM patients who had newly onset of the disease (diagnosed was from one week up to five months) were included in the present study, all the patients were treated with daily replacement doses of insulin. Fifty apparently healthy control subjects underwent the PBL phenotyping. Phenotyping of surface antigens was done by direct Immunoflurocent (IFT) technique using mouse antihuman CD3, CD4, CD8, CD45RA, CD19, and activated markers CD45RO, DR-antigen and CD38. RESULTS: T1DM patients showed a remarkable lowering in CD3+, CD8+, and CD45RA+ cells (p
Recommended Citation
Saleh, Eman Mahdi
(2008)
"Activated Peripheral Blood Lymphocyte Subpopulation in Newly Diagnosed Type 1 Diabetes Mellitus Children,"
Iraqi Postgraduate Medical Journal: Vol. 7:
Iss.
2, Article 3.
Available at:
https://ipmj.researchcommons.org/journal/vol7/iss2/3